@article{JGO18162,
author = {Fatemeh Azarkhazin and Golnaz Asaadi Tehrani},
title = {Detecting promoter methylation pattern of apoptotic genes Apaf1 and Caspase8 in gastric carcinoma patients undergoing chemotherapy},
journal = {Journal of Gastrointestinal Oncology},
volume = {9},
number = {2},
year = {2018},
keywords = {},
abstract = {Background: DNA methylation patterns in cells dysregulation CpG island methylation of genes involved in cancer leads to increased levels of the cancer. Restoration of the apoptotic route in tumor cells of stomach in order for placing Casp8 and Apaf1 genes is a proper approach for new treatments of gastric cancer. The objective of the present study was to investigate the relationship between the pattern of methylation promoter in apoptotic genes of Casp8 and Apaf1 and gastric carcinoma in patients receiving chemotherapy.
Methods: Genomic DNA was extracted from 30 samples of FFPE tumor, normal tissues and blood samples. Hyper-methylation analysis of Casp8 and Apaf1 genes was conducted using MSP method; the results were analyzed through electrophoresis on agarose gel and software spss20.
Results: In this study, methylation rate of Apaf1 gene with (P>0.05) was not signi cant but methylation rate of Casp8 gene with (P},
issn = {2219-679X}, url = {https://jgo.amegroups.org/article/view/18162}
}