Article Abstract

Evaluation of altered expression of miR-9 and miR-106a as an early diagnostic approach in gastric cancer

Authors: Khadije Shirmohammadi, Sareh Sohrabi, Zahra Jafarzadeh Samani, Hosein Effatpanah, Reza Yadegarazari, Massoud Saidijam

Abstract

Background: The role of microRNAs (miRNAs) in cellular processes such as growth, apoptosis, differentiation and proliferation veri es the importance of miRNAs in carcinogenesis. Moreover, levels of miRNAs are dysregulated in cancer cells, so they could be used as novel classes of biomarkers for diagnosing cancer. The oncogenic role of miR-106a and its increased expression have been demonstrated in some cancers. In contrast, there is no consensus for miR-9 expression rate in different cancers. Therefore, this study was done to investigate the role of miR-106a and miR-9 in gastric cancer (GC).
Methods: The current study was performed on 31 GC tissues as case, and 31 healthy adjacent tissues as a control group. Quantitative reverse transcriptase (q-RT) PCR was used for studying the expression rate of both miR-106a and miR-9.
Results: The expression rate of both miRNAs in cancerous tissues was signi cantly higher than healthy adjacent tissues (≈10 folds) (P<0.05).
Conclusions: The results showed that the expression rate of both markers was significantly increased in cancerous tissues. Therefore, they can be suggested as potential biomarkers for cancer diagnosis and prognosis as well as targets for therapy.

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